Cardiotoxic and Lethal Effects of Listeria monocytogenes Hemolysin
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چکیده
منابع مشابه
Cardiotoxic and Lethal Effects of Listeria monocytogenes Hemolysin.
Cardiotoxic and lethal effects of Listeria monocytogenes hemolysin were studied in CD-1 mice injected with varying doses of hemolysin. Intravenous injection of 100 complete hemolytic units (CHU) caused 100% lethality within 4 to 5 min. Doses ranging from 40 to 50 CHU caused death of approximately 50% of the animals. Adrenergic blocking agents and antihistamine failed to protect mice against let...
متن کاملDetection of listeriolysin, the thiol-dependent hemolysin in Listeria monocytogenes, Listeria ivanovii, and Listeria seeligeri.
The listeriolysin gene from a weakly hemolytic but virulent strain of Listeria monocytogenes serotype 1/2a was cloned in Escherichia coli K-12. Recombinants were identified on the basis of their cross-reactivities to hyperimmune antisera raised against streptolysin O and listeriolysin. Low levels of hemolytic activity were detected in crude lysates of strains harboring the listeriolysin gene. I...
متن کاملRole of hemolysin for the intracellular growth of Listeria monocytogenes
Listeria monocytogenes insertion mutants defective in hemolysin production were generated using the conjugative transposons Tn916 and Tn1545. All of the nonhemolytic mutants (hly-) lacked a secreted 58-kD polypeptide, presumedly hemolysin, and were avirulent in a mouse model. An intracellular multiplication assay was established in monolayers of mouse bone marrow-derived macrophages, the J774 m...
متن کاملDETERMINATION OF DOMINANT SEROVARS OF LISTERIA MONOCYTOGENES
Serovars of Lisreria l1Iollocyrogelles were determined. Sera of aborted samples (200) were collected from different hospitals in Tehran and were tested serologically by immunofluorescent antibody methods (IF tests). 137 positive sera were identified. Positive sera were tested against 12 serovars of Listeria l1Iollocytogelles separately. Titers of antibody in patients' sera for all serovars ...
متن کاملPurification, characterization, and toxicity of the sulfhydryl-activated hemolysin listeriolysin O from Listeria monocytogenes.
We purified and characterized an extracellular hemolysin produced by Listeria monocytogenes. Hemolysin production was greatly enhanced by growing bacteria in resin (Chelex)-treated medium. This hemolysin was separated as a homogeneous protein of 60,000 daltons by using thiol-disulfide exchange affinity chromatography. This protein was a sulfhydryl-activated toxin, termed listeriolysin O, which ...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1970
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.1.4.373-379.1970